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Image Search Results
Journal: Communications Biology
Article Title: Bivalent single-domain antibodies show potent mpox virus neutralization through M1R antigen
doi: 10.1038/s42003-025-08494-x
Figure Lengend Snippet: Graphical summary of anti-MPXV VHHs screening. Phage library presenting 3 × 10 9 VHHs was incubated with each biotin labeled-MPXV antigen (either A29L, A35R, B6R, E8L, H3L, or M1R), followed by washing unbound phages. After 3–4 rounds of phage display panning, antigen-bound phages were obtained. Binding of the obtained phage clones to the target antigen was validated by phage ELISA. VHH sequences were identified from the phagemids extracted from the validated clones, and monoclonal VHHs were expressed in periplasm fractions of E. coli BL21.
Article Snippet: Recombinant MPXV antigen proteins A29L (Ser21-Glu110 of QNP13733.1 in E.coli , Sino Biological 40891-V08E) , A35R (Arg58-Thr181 of EPI_ISL_13052264 in HEK293, Sino Biological 40886-V08H) , , B6R (Tyr18-His279 of QNP13760.1 in HEK293, Sino Biological 40902-V08H) , E8L (Met1-Thr275 of Q8V4Y0 in HEK293, RayBiotech 230-30232) ,
Techniques: Incubation, Labeling, Binding Assay, Clone Assay, Enzyme-linked Immunosorbent Assay